2. When determining the catalytic activity of pancreatic lipase from the pancreas of a pig, hydrolyzing sunflower oil, the following data were obtained
S, mM 0.3 0.5 1 2 4 6
Product, mg/min 0.91 1.4 2.3 3.5 4.7 5.25
based on these data, determine graphically, using the Eadie-Hofstee method and the Eisenthal and Cornish-Bowden method, the constants of the Michaelis-Menten equation (Km and Vmax) for this preparation.
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