65. Solving a chemistry problem

8. After the destruction of a proteinaceous antibiotic by the enzyme trypsin and the separation of the resulting mixture on chromatographic paper with the solvent system butanol - pyridine - acetic acid - water (15: 10: 3: 12), amino acids with Rf spots were found, respectively: 0.28; 0.42; 0.49; 0.60; 0.73
In the same solvent amino acids - witnesses have the following R f values: aspargin - 0.65; proline - 0.33; alanine - 0.46; glycine - 0.26; tyrosine - 0.73; serine - 0.81.
Determine the composition of the mixture.