6. During paper chromatography of standard samples of four amino acids - asparagine, glutamine, alanine and proline, the Rf values were 0.14; 0.34; 0.59 and 0.89 respectively. Which of the indicated amino acids are present in the test sample, if during chromatography under the same conditions on the chromatogram with a solvent run of 9.5 cm, two spots were found at a distance from the start line of 3.2 and 8.5 cm.
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