403. Determine the molar mass of hemoglobin according to the following data on the equilibrium centrifugation of its solution:
x, cm 4.16 4.21 4.31 4.36 4.46 4.51
P, wt% 0.398 0.437 0.564 0.639 0.832 0.930
where x is the distance from the axis of rotation of the centrifuge rotor. P is the percentage concentration of protein. Other data: temperature 20 °C, specific partial volume of dissolved protein 0.749 cm3/g, solvent density 1.008 g/cm3, rotor speed 8700 min-1.
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