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The absorbance of 1.00 *10 -4 mol/l of a solution of a certain substance was measured at various wavelengths in a cuvette with an absorbing layer thickness of 0.50 cm. Using the data below, plot the absorption spectrum of the test solution in the coordinates absorbance A - wavelength λ, nm.
Select the analytical wavelength at which the greatest accuracy and sensitivity of determining the substance by the spectrophotometric method is achieved, and at the selected wavelength, calculate the molar extinction coefficient of this substance in solution.
Wavelength, nm Optical density
350 0.090
355-
360 0.085
365-
370 0.075
380 0.085
390 0.110
395-
400 0.190
410 0.325
420 0.460
430 0.600
435 0.635
440 0.630
450 0.575
460 0.475
470 0.360
480 0.257
490 0.212
500 0.165
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